EFFECT OF DIFFERENT PHYTOHORMONES ON PLANT REGENERATION OF AMARYLLIS HIPPEASTRUM

Abstract

Tissue culture of bulbous plants has been successfully established and plantlets areregenerated in large number from this technique. An efficient method for rapid propagation ofAmaryllis hippeastrum L. by In Vitro technique has been developed in this study by usingmicropropagation practice. In this technique explant is inoculated on medium fortified with differentnutrients and growth hormones as well. Scales and meristem of underground bulb used as explantswere cultured on MS medium supplemented with different concentrations and combinations ofcytokinins and auxins. Effect of these phytohormones on plant formation of Amaryllishippeastrumwas studied. Best results for shoot initiation were obtained by using Thidiazurone (TDZ) 10 μM/l +Alpha-Naphthalene acetic acid (NAA) 1.0 mg/l i.e. (90%) frequency of shoot initiation for scalesexplants and 80% frequency of shoot initiation for meristems explants with 3.6± 0.0632 cm of shootlength for both explants. The best results for multiple shoot production were obtained from thecombination of 6-benzyl amino purine (BAP) 5 mg/l + Alpha-Naphthalene acetic acid NAA 0.1 mg/lwith 80% frequency of shoot formation Bulblet formation was promoted in MS basal medium fortifiedwith BAP 4 mg/l and NAA 0.1 mg/l NAA + 60% sucrose. The regenerated plantlets were transferredto different potting media in green house for acclimatization. Best hardening response was obtained in(Vermicompost + Sand). A completely randomized design was used for the experiment. Analysis ofdata was carried out by using COSTAT V.63 software following Duncan’s New Multiple Range Test